Journal: FEMS Microbiology Ecology

Article Title: Drivers of bacterial colonization patterns in stream biofilms

doi: 10.1111/j.1574-6941.2010.00830.x

Figure Lengend Snippet: Fig. 2. CFDA/SE-labeled bacteria (circles) and microbeads (diamonds) remaining in the water column in flumes with 1- (a) or 6- (b) week-old biofilms under laminar (closed symbols) or turbulent (open symbols) flow conditions. Points represent averages of two flumes. First-rate-order models, representing removal by physical factors, are displayed as lines for laminar (continuous line) and turbulent (dashed line) flow conditions.

Article Snippet: Blue fluorescent carboxyl polystyrene latex microbeads (Postnova Analytics GmbH, Germany) (107 beads mL 1) were added to the CFDA/SE-stained cells to test for physical processes, such as transportation.

Techniques: Labeling, Bacteria

Journal: FEMS Microbiology Ecology

Article Title: Drivers of bacterial colonization patterns in stream biofilms

doi: 10.1111/j.1574-6941.2010.00830.x

Figure Lengend Snippet: Fig. 3. Pair cross-correlations between settled CFDA/SE bacteria and microbeads (a, b), settled CFDA/SE bacteria and native biofilm algae (c, d) and settled microbeads and native biofilm algae (e, f). Dotted lines show 95% confidence envelopes. Values significantly 4 1 represent aggregation of the respective tested populations, whereas values o 1 indicate repulsion at the given distance (Fig. 1).

Article Snippet: Blue fluorescent carboxyl polystyrene latex microbeads (Postnova Analytics GmbH, Germany) (107 beads mL 1) were added to the CFDA/SE-stained cells to test for physical processes, such as transportation.

Techniques: Bacteria, Algae

Journal: FEMS Microbiology Ecology

Article Title: Drivers of bacterial colonization patterns in stream biofilms

doi: 10.1111/j.1574-6941.2010.00830.x

Figure Lengend Snippet: Fig. 4. Bivariate analyses (XZ axes) of the CFDA/ SE-cell and microbead point patterns in the cryosections. Graphs in the upper part (a–d) show whether CFDA/SE cells are significantly more or less (dots above or below the confidence envelope, respectively) aggregated than microbeads at a given distance under laminar (a and c) and turbulent (b and d) flow 24 h (a and b) and 36 h (c and d) after their addition to the flumes. Graphs in the lower part (e–h) indicate whether significantly less microbeads or CFDA/SE cells (dots above or below the confidence envelope, respectively) occur in areas where the density of the combined CFDA/SE-cell and microbead pattern is high under laminar (e and g) and turbulent (f and h) flow 24 h (e and f) and 36 h (g and h) after their addition to the flumes. Values significantly 4 1 represent aggregation of the respective tested populations, whereas values o 1 indicate repulsion at the given distance (Fig. 1).

Article Snippet: Blue fluorescent carboxyl polystyrene latex microbeads (Postnova Analytics GmbH, Germany) (107 beads mL 1) were added to the CFDA/SE-stained cells to test for physical processes, such as transportation.

Techniques:

Journal: Results in Immunology

Article Title: Characterization of the liver-macrophages isolated from a mixed primary culture of neonatal swine hepatocytes ?

doi: 10.1016/j.rinim.2014.01.001

Figure Lengend Snippet: Phagocytosis of fluorescence-labeled microbeads by swine macrophage-like cells. After incubation for the indicated periods at 37?°C, cells were fixed, immunostained with an anti-CD172a antibody and observed under phasecontrast/fluorescence microscopy, or analyzed by FACS, as described in 2. Scale bar = 50?µm.

Article Snippet: Fluorescence-labeled polystyrene microbeads (1.0?µm diameter, #17154, Polysciences, Inc., Warrington, PA) were diluted at 1:800 in the growth medium, and added to the isolated macrophage-like cells seeded in eight-well chamber glass slides (10 5 cells/well) or 60?mm non-tissue culture grade plastic dishes (10 6 cells/plate).

Techniques: Fluorescence, Labeling, Incubation, Microscopy